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The graph we made is an accurate representation of our data and how the mass changed due to the sucrose solutions because water was adding to the weight of the dialysis bag. Draw a picture of your hypothesis. Answer key diffusion and osmosis lab answers worksheet. The Scientific Monthly. When solute concentration increases, water potential decreases. In this case, you can imagine that the solution is less concentrated than the cell's cytoplasm, causing water from the solution to flow into the cell. Atomic formula: I or I2.
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If we determine the molarity of the sucrose solution that will help produce equilibrium between the solution and the contents of the potato cell, we can determine solute potential: Ψs – iCRT, where i is ionization constant, C is molar concentration of sucrose per liter at equilibrium, R is pressure constant (0. Moreover, perfume fragrance also spreads throughout the room. Enough of the 40% sucrose solution to cover the bag. Diffusion osmosis post lab KEY.docx - Diffusion and Osmosis Post-Lab Directions: Answer the following questions within your group. Please be sure that | Course Hero. Sucrose bag because the concentration of sucrose was larger and thus, contained less water then.
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For activity A, the hypothesis stated that if we added glucose-starch solution to a dialysis tubing bag and submerged it in a cup of distilled water and IKI solution then glucose will leave the dialysis bag through the pore into the IKI and distilled water solution through diffusion. Imbibition||Hydrophite substances. However, as stated by Hawthorn, a semi-permeable membrane is not absolutely necessary for the. Diffusion and Osmosis Questions - Practice Questions of Diffusion and Osmosis with Answer & Explanations. A free energy-gradient for water must be present in cells for osmosis to occur. For each 15-min interval, record the total weight of each bag in table 2. If necessary, the results are supported by illustrations (tables, graphs, figures), which present the original material or evidence in a collapsed form. Osmosis and diffusion are chemical phenomena occurring due to differences in concentration. Procedure 3: Determine the Concentration of Solute in living plant cells. Column I||Column II|.
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Changes in Weight of Dialysis Bags used as Cellular Models. Add 10 ml of starch solution to the other dialysis tube. Record the colors of both the dialysis bag and the glass. Factors affecting Water potential and Solute potential. Answer key diffusion and osmosis lab answers key. Next, we dipped a new glucose test strip into the glucose/starch solution. It is a simple lab where students do very little except watch the process and record data and information. Osmosis is an important biological concept. Do not describe all the work, only the most essential details, by volume this part of the work amounts to several sentences, at most half a page; - Your next assignment is to write the theoretical part. Remove the glasses from the refrigerator, and gently put the eggs on a plate.
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To set it up, you will need plastic bags, iodine, water, and corn starch. Place bag A in empty beaker and will with just enough 50% sucrose to cover the bag. This is the main section, which aims to prove a working hypothesis by analyzing, synthesizing, and explaining the data. Iodine is an indicator that turns blue in the presence of starch. Larger molecules into smaller ones, or the reverse.
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HypotonicIn which solution did the potato slices feel most flaccid? Procedure 2: Oberve Osmosis in Different Conditions and observe the effect of steepness in concentration graident on the rate of osmosis. For each sucrose solution, we poured 160-170 mL of distilled water into a cup. Permeable membrane from a higher concentration to a lower concentration (Campbell, pg. In this case, water moves around to different areas based on a concentration gradient, i. e. solutions which have different concentrations of dissolved particles (solutes) in them. The farmer using the sea water from the Mediterranean Sea in problem 7 should be advised because the salt water will pull the remaining water out of the wheat plant cells because of its hypertonic nature. Use gloves and goggles as precaution. That is, your task is to apply the laws of chemistry, biology to the results obtained and compare. Biology formal lab report on osmosis and diffusion. What's one thing that all of these processes have in common? 9% Salt solution increased in weight. 1g of water and the bag of water in the 40%. Calculate the change in weight and change in time (total time) for each cylinder and record in Table 3. Its peculiarity is a generalization, abstractness, and an abundance of special scientific terms.
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With the baggie in place, you will need to prepare enough beakers for your entire class. Below, under the type of work, indicate the topic of the work but without quotes. Motvaton some are biological some psychological Pyramid of Maslow needs. C) Facilitated diffusion. Furthermore, the entire data may not be so reliable because the 5% salt solution had the highest rate of diffusion, followed by 10% and 15% salt solution (table 3). Measuring cup (1 cup). In the experiment, the sucrose. It is also an example of diffusion. Answer key diffusion and osmosis lab answers manual. Color in Iodine: Bluish. Next, we placed the dialysis bags one through four in there own separate beakers. It depends on the molar concentration of the solute particles in the solution and the surrounding temperature. This should take about 2-3 days.
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Practise Questions on Diffusion and Osmosis. Since the solute concentration of outside solution is known, one can determine the concentration of solute in potato cells by the change in weight after it reaches equilibrium. The bag that contained the most weight was the 60% sucrose bag due to the fact that it had the. Which substance diffused across the membrane in beaker #2? When making a diffusion experiment, factors such as temperature, molecular weight, electrical charge, and substance concentration must be considered. White vinegar (about 3 cups). After measuring the weight, we covered the potato sections back up to prevent evaporation of the solution. After a 24 hour period, we recorded the ambient temperature given by the teacher in Table 3. Further questions/other comments.
If the starch moved, the outside of the dialysis bag would have also turned blue because when Iodine reacts with starch, it turns blue. Osmosis is a selective form of diffusion. Bag A was in a hypertonic solution, so decrease in weight is expected since water will move to the outside which has more solutes to balance. We can classify osmotic solutions into three kinds. A value of -9 will be assigned for variable Ψs. Place bag B, C, and D in large beaker filled with 1% sucrose. Answer: Various factors that affect the rate of diffusion are mentioned below. Movement of iodine in solution. Experiment, the dialysis tubing acted as the selectively permeable membrane. We recorded the initial color of the glucose/starch solution in Table 1. One striking trend seen in Table 1 is the consistent increase in 6H binding.
Only plot total weight, and not the change in weight data. GlucoseWhich solute did not diffuse through the dialysis tube membrane - starch or glucose? S tarch, a large molecule, was unable to move while iodine, sodium hydroxide, and phenolpthaleine, which are small molecules, were able to move, supporting the hypothesis that big molecules will not be able to cross the membrane barrier (dialysis bag) while small molecules can. If the molecules are headed toward an open pore in the cell membrane, it may pass through the pore or rebound depending on its size to the pore. Draw a picture of each system below. After measuring the weight of all the potato sections, we placed all of the potatoes into the labeled cups.
Take dental floss and tie it around one end of the dialysis tube. My lab partners and I placed clamps on. By definition, a hypertonic solution is one that causes a cell to shrink. Also, steeper gradient increased rate of osmosis demonstrated by the fact that Bag D increased faster than Bag C (fig. Light corn syrup (about 1 ¼ cups).